🔍 Research Essentials | Experimental Insights into the Macrophage Transfection Tool AD600150
🌟 Key Highlights: ZETA Life''''s AD600 transfection reagent played a starring role in this high-impact study published in Frontiers in Immunology (a top journal)!

🧪 Which cells were transfected?
✔️ THP-1 cells (human origin)
• Six siRNAs were used to knock down long non-coding RNAs (lncRNAs; e.g., P33714). It was discovered that their murine homolog, Gm28309, regulates inflammation.
✔️ RAW264.7 cells (murine origin)
• Overexpression of the Gm28309 plasmid combined with miRNA mimics/inhibitors (miR-3068-5p) confirmed that Gm28309 modulates the NF-κB pathway via "sponge-like" miRNA sequestration.

💡 Technical Highlights
• Dual-luciferase reporter assays provided clear validation of target interactions ✔️
• Transfection efficiency was rigorously controlled by qPCR, ensuring robust and reliable data 👌

👉 Researchers, take note: For studies on macrophage inflammatory mechanisms or competing endogenous RNA (ceRNA) pathways, AD600150 is a top choice. The original article is available in Frontiers in Immunology (2020; DOI: 10.3389/fimmu.2020.581517).