Product Name: Goat Anti-Mouse IgG Antibody (H&L) HRP
Target Species: Mouse IgG
Tested applications: IHC-P, WB, ELISA
Immunogen: Mouse IgG, whole molecule
Conjugation: HRP
Applications
WB:1/3000-1/20000
IHC:1/100-1/2000
ELISA : 1/5000-1/10000
Working dilutions are highlighted in the table below,use at an assay dependent dilution.
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They
eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and
sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on
film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are
easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are
utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates
have a very high turnover rate, yielding good sensitivity with short reaction times.