Sirius DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA and siRNA , then transports the complex insect cells.

Storage:  Store at 4°C (Don’t Freezing).

Functional analysis:
Cos 7 cells are transfected in 96 well plates with a reporter gene vector DNA, using 0.2-0.5 μl Sirius Transfection Reagent and 0.1 μg DNA or 0.25 μl 

Sirius  Transfection Reagent and 0.1 μg DNA. Reporter gene activity is monitored via a colorimetric test against a reference lot. Activity must be 70% - 90%

 compared to a reference lot in at least one of the tested amounts, listed above.

Procedure Details
Component	96-well	24-well	12-well	6-well
Adherent cells	1–4 × 104	0.5–2 × 105		0.25–1 × 106
Opti-MEM® Medium	25 μL × 4	50 μL × 4	100 μL × 4	150 μL × 4
Sirius  Transfection Reagent	1, 1.5, 2, 2.5 μL	2, 3, 4, 5 μL	4,6,8,10ul	6, 9, 12, 15 μL
Opti-MEM® Medium	125 μL	250 μL	500ul	700 μL
DNA (0.5–5 μg/μL)	2.5 μg	5μg	10ug	14 μg
Diluted DNA Total	25 μL	50 μL	100ul	150 μL
Diluted Sirius Transfection Reagent	25 μL	50 μL	100ul	150 μL
Incubate for 5 minutes at room temperature.
Component	96-well	24-well	12-well	6-well
DNA-lipid complex per well	10 μL	50 μL	100ul	250 μL
Final DNA used per well	100 ng	500 ng	1000ng	2500 ng
Final Sirius Transfection Reagent used per well	0.2–0.5 μL	1.0–2.5 μL	2.0-5.0ul	5.0–12.5 μL
Incubate cells for 1–3 days at 37°C. Then analyze transfected cells.

Cytotoxicity analysis:
Cell growth is assessed using the Cell Counting Kit (K009,ZETA LIFE Inc.) which is an indirect measure of cytotoxicity. The remainder of cells from functional

 analysis is tested for viability.

Sirius DNA Transfection Reagent complexes must be made in serum-free medium such as Opti-MEM Reduced serum Medium and can be added directly to cells in 

culture medium, in the presence or absence of serum .

It is not necessary to remove complexes or change and add medium after transfection.The amount of Sirius Transfection Reagent required for successful 

transfection varies depending on the cell type and passage number. Start any new transfection by testing the recommended four concentrations of Sirius DNA  to 

determine an optimum amount.

Sirius DNA Transfection Reagent is a proprietary formulation for transfectin nucleic aicids into a wide range of eukaryotic cells . It is designed to transfect a 

wide range of eukaryotic cells including insect cells and many cell lines not transfected well by other reagents （e.g .,MCF-7, RAW 264.7, PC-3, HeLa, MA-10, 

HepG2, SH-SY5Y, A7r5, STO, SCC-61, STSAR-90, SQ20B, T98）.

Note: For the most up-to-date list of cell types that have been successfully transfected with Sirius DNA Transfection Reagent for life science research only.