ECM endothelial cell culture medium is a sterilized liquid medium containing essential and non essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors (ecgsecgf), trace minerals and low concentration fetal bovine serum (5%). The buffer system of endothelial cell culture medium (ECM) is bicarbonate, and the pH value is 7.4 after equilibrium in a cell incubator containing 5% CO2. The formulation of endothelial cell culture medium (ECM) can selectively promote the proliferation and growth of normal human microvascular endothelial cells in vitro, and provide quantitative and quality assurance for their nutritional balance.