Tyrosinase (EC1.14.18.1) is a kind of monophenol monooxygenase, which is a bi-functional copper-containing glycoprotein, widely existing in plants,

Yeast and animal tissue. Tyrosinase is a key enzyme in the synthesis of melanin, and is the main factor causing enzymatic Browning of fruits and vegetables, as well as insects

Immunity and growth have important effects

Tyrosinase catalyzed l-dopa to produce dopa pigment, which had a characteristic absorption peak at 475nm, and then measured the activity of tyrosinase.