This kit is designed by double antibody sandwich ELISA method. The anti-mouse ALB antibody is coated on the elisa plate, and the mouse ALB in the sample or standard product will bind to the coated antibody, and the free components are washed away. Biotinylated anti-mouse ALB antibody and horseradish peroxidase labeled avidin were successively added. The anti-mouse ALB antibody binds to the mouse ALB bound to the coated antibody, and the biotin specifically binds to avidin to form an immune complex in which the free components are washed away. Color substrate (TMB) is added, TMB is blue under the catalysis of horseradish peroxidase, and becomes yellow with the addition of termination solution. The CONCENTRATION of ALB in the sample is calculated by drawing a standard curve.